【摘要】 甜菊醇是新型甜味剂甜菊糖在人体内的代谢产物,在酸性环境中可重排成异甜菊醇,它们都是重要的药物合成中间体。研究甜菊醇和异甜菊醇与DNA的作用方式有助于理解其药理或细胞毒性。以小牛胸腺DNA(CT-DNA)为模板,通过考察甜菊醇和异甜菊醇对CT-DNA熔点和粘度的影响,以及甜菊醇和异甜菊醇与CT-DNA体系紫外吸收的变迁,初步推断甜菊醇和异甜菊醇都是以沟槽方式与CT-DNA结合;异甜菊醇与CT-DNA的作用比甜菊醇与CT-DNA作用强。更多还原

【Abstract】 This work reported the interaction between steviol or isosteviol and CT-DNA by observing the changes on UV,viscosity and melting point of the CT-DNA before and after the interaction. Based on the results,it could be inferred that the binding of steviol and isosteviol with CT-DNA is a groove binding,and the interaction between isosteviol and CT-DNA is stronger than that of steviol and CT-DNA.更多还原