【摘要】 建立了湖南黑茶中水溶性成分的液相色谱–离子阱串联飞行时间质谱(LC–IT–TOF MS)快速分离和确证方法。实验用90℃热水恒温提取湖南黑茶样品中水溶性成分,采用液相色谱–离子阱串联飞行时间质谱仪对其中的水溶性提取物进行分离测定。利用准确质量数匹配和自建质谱数据库检索,结合保留时间、多级质谱、特征碎片离子等信息,对湖南黑茶中的43种水溶性化合物的进行了初步确证。该法可得到黑茶独特滋味品质的特征成分,为湖南黑茶品质评价和质量控制提供了一种新的技术手段。更多还原

【Abstract】 The liquid chromatography–ion trap time of flight mass spectrometry(LC–IT–TOF MS)method for rapid separation and identification of water–soluble components in Hunan dark tea was established. The samples of Hunan dark tea were extracted by hot water at 90℃. The water–soluble extracts were analyzed by liquid chromatography–ion trap time of flight mass spectrometry(LC–IT–TOF MS). The 43 compounds were isolated and identified simultaneously by using accurate mass matching and self-established mass spectrometry database combined with retention time,multiple-stage ion mass spectral,characteristic fragment ions. Through identification of the characteristic component of dark tea’s unique taste quality, a new technical means for Hunan dark tea quality evaluation and control was provided.更多还原