【摘要】 目的:研究克癃胶囊对双氧水诱导的PC-3细胞氧化应激反应的保护作用。方法:体外培养PC-3细胞,建立双氧水诱导PC-3细胞氧化应激的模型,给予克癃胶囊进行干预。分为4组:正常组、模型组（H₂O₂ 100μmol/L）、阴性对照组（tBHQ50μmol/L+H₂O₂100μmol/L）、克癃胶囊组（克癃方100μg/m L+H₂O₂ 100μmol/L）以DCFH-DA为荧光探针,利用流式细胞仪检测细胞内活性氧自由基（ROS）含量,利用化学显色法测定PC-3细胞内还原型谷胱甘肽（GSH）含量,评价细胞氧化应激水平。结果:ROS、GSH水平模型组与正常组比较,阳性对照组、克癃胶囊组与模型组比较,差异均有统计学意义（P<0.01）;ROS水平阳性对照组与克癃胶囊组比较,差异有统计学意义（P<0.01）,但两组GSH水平比较,差异无统计学意义（P>0.05）。结论:克癃胶囊对双氧水诱导的PC-3细胞氧化应激具有保护作用。更多还原

【Abstract】 Objective: To investigate the protective effect of Kelong capsules against the oxidative stress response of PC-3 cells induced by hydrogen peroxide. Methods: PC-3 cells were cultured in vitro and treated with hydrogen peroxide to establish an oxidative stress model. Kelong capsules were given for intervention. PC-3 cells were divided into normal group,model group( H2O2100μmol/L),positive control group( t BHQ 50μmol/L + H2O2100μmol/L),and Kelong capsule group( Kelong capsules 100μg/m L + H2O2100μmol/L). With DCFH-DA as the fluorescent probe,flow cytometry was used to measure the reactive oxygen species( ROS) production in PC-3cells. Chemical development was used to measure the level of glutathione( GSH) in PC-3 cells and to evaluate intracellular oxidative stress. Results: The ROS and GSH levels showed significant differences between the model group and normal group and between the positive control group,Kelong capsule group,and model group( P < 0. 01). There was a significant difference in ROS level between the positive control group and Kelong capsule group( P < 0. 01),but no significant difference was found in GSH between the two groups( P > 0. 05). Conclusion: Kelong capsules can protect PC-3 cells against oxidative stress induced by hydrogen peroxide.更多还原