【摘要】 目的:观察木犀草素体外对感染甲型流感病毒（H₁N₁）的人肺癌细胞株（A549细胞株）的效果,探讨木犀草素在体外环境中对H₁N₁感染A549细胞的作用。方法:采用细胞病变效应（CPE）预估木犀草素抗病毒活性指数,计算出最大无毒浓度（TC0）、半数毒性浓度（TC50）;检测不同时间和作用方式时木犀草素的吸光度,找出木犀草素抗病毒最佳作用方式,并计算出半数抑制浓度（IC50）以及治疗指数（TI）。结果:木犀草素的半数中毒浓度为39.81μg·m L^-1,最大无毒浓度为25.00μg·m L^-1;浓度在12.50²5.00μg·m L^-1,木犀草素作用48⁶0 h,对H₁N₁所致的细胞病变作用有明显的抑制作用。病毒感染后2 h加入25.00μg·m L^-1浓度的木犀草素,抗病毒有效率最高为（87.00±1.71）%。计算该条件下木犀草素半数抑制浓度为11.282μg·m L^-1,治疗指数为3.528。结论:木犀草素在一定条件下具有抗H₁N₁的作用。更多还原

【Abstract】 Objective: To observe the role of Luteolin after the infection of influenza virus（H₁N₁） in human lung cancer cell line（A549 cell line）. Methods: The maximum of non-toxic concentration（TC0） and the median toxic concentration（TC50） were detected by the cytopathogenic effect（CPE） of cells. Furthermore, The median inhibitory concentration（IC50） and treatment indexes（TI） in the best mode were calculated by Probit regression.Results: The median toxic concentration of Luteolin was 39.810 μg·m L^-1, and maximum non-toxic concentration was 25 μg·m L^-1; A significant inhibitory effect was shown when added luteolin from 12.5 to 25.0 μg·m L^-1after48-60 h compared with the model without the drug. In addition, the best method against viral infection was adding Luteolin at 2 after cells infected and the best survival rate was（87.00±1.71） % with 25.0 μg·m L^-1of Luteolin. Under this condition, IC50 was 11.282 μg·m L^-1, and TI was 3.528. Conclusion:Luteolin possesses the effect of anti-H1 NI virus in vitro.更多还原