【摘要】 目的:观察腰5(L5)脊神经结扎(spinal nerve ligation,SNL)致慢性疼痛大鼠接受背根神经节脉冲射频(pulse radiofrequency,PRF)治疗后痛觉行为学的变化,检测脊髓背角自噬相关基因LC3和自噬受体蛋白P62的表达情况,研究PRF是否能通过调节脊髓背角神经元自噬水平发挥镇痛作用。方法:36只SD大鼠随机分为假手术组(Sham组)、SNL组(行左侧L5脊神经的暴露和结扎)和SNL+PRF组(建立SNL模型后即刻给予1次同侧背根神经节PRF治疗)。分别于手术前1 d及术后1,3,7,14,28 d观察各组大鼠机械刺激缩足反应阈值(paw withdrawal mechanical threshold,PWMT)改变,Western印迹检测自噬相关基因LC3和细胞自噬受体蛋白P62在大鼠损伤侧相应节段脊髓背角的表达改变。结果:与Sham组相比,SNL组大鼠在术后各时点PWMT均明显下降(P<0.05);与SNL组相比,SNL+PRF组大鼠在PRF治疗术后第1天即出现PWMT的升高,并持续28 d(P<0.05);与Sham组相比,SNL组大鼠在术后7 d脊髓背角LC3-II和P62蛋白表达明显升高(P<0.05);与SNL组相比,SNL+PRF组大鼠在术后7 d脊髓背角LC3-II和P62蛋白表达明显下调(P<0.05)。结论:SNL慢性疼痛大鼠接受背根神经节PRF治疗后出现疼痛行为学改善,PRF治疗可能通过上调脊髓背角神经元自噬水平发挥镇痛作用。更多还原

【Abstract】 Objective: To detect the effect of pulse radiofrequency（PRF） treatment on the neuropathic pain established by L₅-spinal nerve ligation（SNL） on rats, and to investigate if PRF treatment would affect the expression of autophagy related protein LC3 and autophagy related receptor P62 at the dorsal horn.Methods: A total of 36 male Sprague-Dawley rats were randomly divided into 3 groups: a Shamgroup, a SNL group, and a SNL+PRF group. The 50% paw withdrawal mechanical threshold（PWMT） was detected at 1 day before and 1, 3, 7, 14 and 28 days post-operation by using Von-Frey filaments. The autophagy related protein LC3 and autophagy related receptor P62 were investigated by Western blot.Results: Compared with the Sham group, the PWMT significantly decreased in the SNL group at each time points（P<0.05）; in SNL+PRF group, PRF treatment could elevate the PWMT at the 1st day post-operation and lasted for 28 days（P<0.05）. What’s more, SNL could elevate the LC3-II and P62 levels at the 7th day post-operation（P<0.05）, which were decreased by the PRF treatment（P<0.05）.Conclusion: PRF treatment could improve SNL-induced the neuropathic pain, which might be partly due to the regulatory effects on the autophagy levels at the spinal dorsal horn.更多还原