We used loach as experimental materials to confirm the acute toxicity of atrazine firstly.In order to correctly estimate the environmental toxicity of atrazine and the effects on aquatic organisms reproductive development,we explored the long-term treatment effects of atrazine on gonad shape and sex differentiation related gene expression by histology and real-time quantitative PCR(qRT-PCR).On the basis of acute toxicity experiment,the experimental results showed that:24h,48hand96hLC50 of atrazine were 31.6...
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