【摘要】 为研究MeHDS09基因的结构与功能,及其在木薯抵御不同逆境胁迫中的作用。笔者基于木薯转录组数据及全基因组公共数据库,采用RT-PCR手段获得2 151 bp,具有完整ORF的MeHDS09基因,其编码716个氨基酸,蛋白质相对分子质量为81.3 k D;生物信息学分析结果显示,该蛋白无信号肽位点,具有线粒体靶向肽,主要定位于线粒体。序列分析结果显示,MeHDS09基因序列与大豆中增强抗病性的蛋白2基因具有较高的同源性;其上游启动子区序列除含有启动子核心元件外,还具有抗病、激素应答等反应元件,此结果暗示MeHDS09基因的表达可能受外界环境因素的影响。进一步利用qRT-PCR分析表明:MeHDS09基因对GA/IAA反应不灵敏;低温、ABA、JA处理可显著诱导木薯中MeHDS09的表达,而高温和SA处理则显著抑制了MeHDS09的表达。综上所述,MeHDS09可能在木薯抵御低温等逆境胁迫反应中起作用。本研究结果为研究MeHDS09基因的生物学功能及其应用奠定了基础。更多还原

【Abstract】 The gene MeHDS09 was cloned from cassava to analyze its structure and functions in tissue cultured plants under different abiotic stresses. MeHDS09 was isolated from cassava plant using RT-PCR and had 2 151 bp open reading frame encoding 716 amino acids according to the transcriptome data and genome-wide public sequences on cassava. The MeHDS09 encoded protein was 81. 2 k D in molecular weight,and did not contain the signal peptide site. Subcellular localization prediction showed that MeHDS09 contained mitochondrial targeting peptide and was mainly localized in mitochondrion. Comparison of sequences similarity with reported genes sequences in Genbank showed that MeHDS09 might have higher homology with Protein 2 in soybean,which was related to enhancement of plant disease resistance. The promoter sequence analysis showed that the upstream promoter sequence of the MeHDS09 contained not only promoter core elements but also hormone responsive elements in response to disease resistance and hormones. This indicated that the expression of MeHDS09 might be influenced by the external environment factors. Further analysis with qRP-PCR showed that the MeHDS09 was not sensitive in response to GA/IAA,and that the cold,ABA and JA treatments induced significant expression of MeHDS09 in cassava,while the hot and SA treatments inhibited obviously expression of the MeHDS09. The results revealed that MeHDS09 might play a role in resistance to abiotic stresses in cassava,such as low temperature.更多还原