【摘要】 该研究旨在比较良种肉羊5周龄、13周龄羔羊激素处理后卵巢卵泡发育及体外胚胎发育的影响。通过FSH和孕马血清促性腺激素(PMSG)处理,比较卵巢上2~8 mm卵泡的数量及体外胚胎的发育情况。结果表明:5周龄羔羊卵巢发育的卵泡数和采集到的卵母细胞数(58.6±1.9、47.4±4.2)都高于13周龄羔羊卵泡数和卵母细胞数(14.4±4.1、11.6±1.7),且差异显著(P<0.05)。5、13周龄羔羊卵母细胞受精后的卵裂率(60.0%±1.9%、61.6%±5.2%)及囊胚率(27.4%±2.1%、28.2%±3.7%),两者间差异不显著。更多还原

【Abstract】 The aim was to compare the effects of hormone treatments on the ovarian follicular development and in vitro embryo development between 5-week and 13-week old lambs. FSH and pregnant mare serum gonadotropin(PMSG) treatment were used to compare the number of 2-8 mm follicles in the ovary and the in vitro embryo development. The results showed that: the 5-week old lambs had larger follicular numbers and collected oocytes numbers(58.6 ±1.9, 47.4 ±4.2) than the 13-week old lambs(14.4±4.1, 11.6±1.7), and the difference was significant(P<0.05).However, there was no significant difference in the cleavage rate(60.0±1.9%, 61.6±5.2%) and blastocyst rate(27.4±2.1%, 28.2±3.7%) of the fertilized oocytes between the 5-week and 13-week old lambs.更多还原