【摘要】 目的探讨不同浓度的二氯乙酸钠（DCA）对人骨肉瘤MG63细胞活性、周期及凋亡的影响。方法用含质量分数10%胎牛血清的RPMI-1640培养基培养MG63细胞,不同浓度DCA作用于对数生长期的细胞,CCK8试剂盒检测50、100、200μg·mL^-1DCA处理24 h、48 h后细胞增殖情况;流式细胞术检测100、200μg·mL^-1DCA-Na作用24 h、48 h后细胞周期和凋亡情况;qRT-PCR检测100、200μg·mL^-1DCA处理后细胞周期相关基因CDK2的变化。结果 CCK8检测结果显示50、100、200μg·mL^-1DCA均能抑制MG63细胞的生长,且有时间和浓度依赖性;不同浓度的DCA-Na分别干预MG63细胞不同时间后都明显阻滞细胞于G2/M期,且细胞凋亡率增加（P均<0.001）;不同浓度的DCA干预MG63细胞后,细胞中的CDK2mRNA的相对表达量明显降低（P均<0.001）。结论 DCA通过抑制CDK2使MG63细胞阻滞于G2/M期,进而诱导细胞凋亡,抑制细胞的生长。更多还原

【Abstract】 Objective To determine the effect of sodium dichloroacetate( DCA) on human osteosarcoma cell line MG63 in vitro activity,cell cycle and apoptosis. Methods The MG63 cells were cultured with RPMI-1640 medium including 10% of fetal bovine serum as usual,and treated with different concentration DCA. Then CCK8 kit was used to observe growth suppression of cells under different concentrations of DCA at 24 h,48 h; Flow cytometry assay was used to determine the cycle and apoptosis of the cells that were treated by different concentrations of DCA for 24 h,48 h,respectively. Finally,qRT-PCR was used to determine the mRNA relative expression of cell cycle related gene CDK2 after DCA invention. Results Different concentration of DCA inhibited cell growth and proliferation in a time-and dosedependent manner; different concentration of DCA-Na acting on different time respectively arrested cell cycle G2/M phase,and improved apoptosis( P < 0. 001); and decreased the expression of CDK2 gene( P < 0. 001). Conclusion DCA can arrest G2/M cell cycle phase through inhibiting activity of CDK2,and then DCA induce apoptosis of MG63 cells and inhibit the growth of tumor cells.更多还原