【摘要】 目的探讨缺氧环境下脂氧素A₄（lipoxin A₄,LXA₄）对肝癌细胞增殖的影响。方法采用小鼠肝癌细胞H22接种构建小鼠肝癌皮下移植瘤模型,观察LXA₄对肿瘤生长的影响。采用免疫组织化学法检测细胞增殖核抗原Ki-67和缺氧诱导因子-1α（hypoxia-inducible factor-1α,HIF-1α）表达。四甲基偶氮唑盐比色法检测细胞增殖水平。结果在接种肝癌细胞H22的7¹5 d,小鼠皮下可见清晰肿瘤包块生长;在各相应时间点,给予LXA₄的小鼠肿瘤体积均明显缩小（P<0.05）,同时明显降低了肿瘤组织内部Ki-67和HIF-1α表达。体外培养发现200 nmol/L LXA₄可明显抑制缺氧条件下人肝癌细胞HepG2的增殖。结论 LXA₄能抑制缺氧条件下肝癌细胞的增殖。更多还原

【Abstract】 Objective To explore the effect of lipoxin A₄（LXA₄） on the proliferation of human hepatocellular carcinoma（HCC） under hypoxic condition.Methods Ectopic HCC mice model was established by injection H22 cells subcutaneously.Immunohistochemistry assay was applied to detect the protein expression of Ki-67 and hypoxia-inducible factor-1α（HIF-1α）.HepG2 cells were cultured under 1%O₂ to establish a hypoxic condition.3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide（MTT） assay was used to measure cell proliferation rate.Results In 7 to 15 days after H22 cell transplantation,the subcutaneous tumor in mice could be observed.At all the time points,the tumor volume of LXA₄ mice was significantly reduced（P<0.05）.At the same time,LXA₄ significantly decreased the expression of Ki-67 and HIF-1α inside tumor tissue.In vitro study showed that 200 nmol/L LXA₄ obviously inhibited HepG2 proliferation under 1%O₂.Conclusion LXA₄ could inhibit the HCC growth under hypoxia.更多还原