To study the effect of biochar amendment on bacterial community structure and diversity in straw-amended soils,the bacterial communities in the straw-amended soils with( SBW) and without( SW) biochar were analyzed by nested PCR-DGGE technique combined with sequencing. The bacterial DGGE profiles and genetic diversity index revealed that the biochar amendment promoted the variation of the DGGE band position and gray value as well as the evenness of bacterial communities,but made insignificant difference( p >...
【基金】
国家自然科学基金(No.41103067,41273095)~~
【更新日期】
2017-02-17
【分类号】
S154.3
【正文快照】
1引言(Introduction)秸秆还田是当今世界范畴内改善农田生态环境的重要措施之一,它有利于改善土壤结构、提高土壤有机质含量和促进农业生态系统良性循环(Witt et al.,2000).秸秆还田过程中,秸秆在土壤中分解主要是一个微生物代谢过程.土壤微生物对秸秆腐解及其营养元素释放具有