【摘要】 采用微生物技术解决城市景观水体自我净化能力差和富营养化严重问题是生物技术重要方法之一,本实验从安徽农业大学小池塘底泥中选育出具有超强水质净化能力的3株菌株,并标记为p1、XLX1和XLY1,通过形态、生理生化试验和基因序列比对分析,分别鉴定为沼泽红假单胞菌（Rhodopseudomonas palustris）,解糖假苍白杆菌（Pseudochrobactrum saccharolyticum）和热带产朊假丝酵母（Candida tropicalis）,并将其与本实验室已获得乳酸菌,施氏假单胞杆菌和枯草芽孢杆菌进行复配和净化效果优化的研究.结果表明,6株菌株之间无拮抗性,其最佳复配比例为p1∶HJ132∶XLY1∶XLX1∶HJ025∶HJ136=1.5∶0.5∶1∶1.5∶0.5∶1,复配后的水质净化菌剂在30℃,初始pH值为6.5,接种量为9%,培养时间为72 h,氮源为酵母膏+NH₄Cl,碳源为淀粉时净化效果最好,氨氮和COD的去除率分别达到了97.3%和77.7%.更多还原

【Abstract】 Using microorganism technology to address problems such as poor self-purification capability and eutrophication of urban landscape water is one of the most important issues in biotechnology. Three strains with strong water purification capacities were bred from a small pond in Anhui Agriculture University and named p1,XLX1,and XLY1. According to their morphology,physiological tests, biochemical tests, and gene sequence analysis, they were identified as Rhodopseudomonas palustris,Candidatropicalis and Pseudochrobactrum saccharolyticum. These three strains were mixed with Lactobacillus, Pseudomonas stutzeri and Bacillus subtilis. which our laboratory had obtained,and studied on the ptimization of the purification effect. Our results showed that there was no antagonism between the six strains. Furthermore,the optimal compound proportion was p1∶ HJ132∶ XLY1∶ XLX1∶ HJ025∶ HJ136 = 1. 5∶ 0. 5∶ 1∶ 1. 5∶ 0. 5∶ 1 under the following conditions:30 ℃,an initial pH value at 6.5,9% inoculation,72 hour cultivation,yeast extract+NH₄Cl as thesource of nitrogen,and starch as the source of carbon. These conditions provided an ammonia nitrogen removal rate of 97.3% and COD removal rate of 77.7%.更多还原