【摘要】 目的探讨Wnt/β-catenin通路中Wnt通路抑制因子(Wnt inhibitory factor-1,WIF-1)、β连环蛋白(β-catenin)和细胞周期蛋白(cyclin D1)在甲状腺乳头状癌(papillary thyroid cancer,PTC)中的表达及意义。方法选取PTC患者60例,留取癌灶组织和癌旁组织标本。检测WIF-1、β-catenin和cyclin D1mRNA和蛋白表达,并分析WIF-1mRNA与β-catenin和cyclin D1mRNA的相关性。结果 PTC组织WIF-1mRNA表达水平和蛋白表达阳性率均显著低于癌旁组织,β-catenin、cyclin D1 mRNA表达水平和蛋白表达阳性率均显著高于癌旁组织(P<0.05)。PTC淋巴结转移组WIF-1mRNA表达水平和蛋白表达阳性率低于无淋巴结转移组,β-catenin、cyclin D1mRNA表达水平和蛋白表达阳性率高于无淋巴结转移组(P<0.05)。不同性别、年龄、肿瘤直径、病灶数目和是否包膜侵犯PTC中WIF-1、β-catenin、cyclin D1mRNA表达水平和蛋白表达阳性率差异无统计学意义(P>0.05)。WIF-1mRNA与β-catenin和cyclin D1 mRNA表达呈负相关。结论WIF-1基因在PTC中表达下调促进了β-catenin蛋白在细胞质中累积,导致靶基因cyclin D1过度激活。WIF-1下调和(或)β-catenin、cyclin D1上调在PTC的发生和转移中发挥促进作用。更多还原

【Abstract】 Objective To study expression and significance of Wnt inhibitory factor-1(WIF-1),β-catenin and chclin D1 in Wnt/β-catenin signal transduction pathway in papillary thyroid cancer(PTC).Methods Sixty cases of PTC were selected and the specimens of cancerous foci and adjacent tissues were taken.The expression of WIF-1,β-catenin and cyclin D1 mRNA and protein were detected,and the correlation between WIF-1mRNA andβ-catenin,cyclin D1 mRNA was analyzed.Results The expression levels of WIF-1 mRNA and positive rates of WIF-1protein expression in PTC tissues were significantly lower than those in paracancerous tissues(P<0.05),while expression levels ofβ-catenin,cyclin D1 mRNA and protein in PTC tissues were on the contrary(P<0.05).And the expression levels of WIF-1mRNA and protein in PTC lymph node metastasis group was lower than those without lymph node metastasis,but the expression levels ofβ-catenin,cyclin D1 mRNA and protein in PTC lymph node metastasis group were on the contrary(P<0.05).There were no statistically significant differences in the expression levels of WIF-1,β-catenin,cyclin D1 mRNA and positive rates of protein in PTC tissues in different sex,age,tumor diameter,number of lesions and whether or not the capsule was invaded(P>0.05).Expression levels ofβ-catenin,cyclin D1 mRNA were negatively correlated with those of WIF-1mRNA(P<0.05).Conclusion The down regulation mechanism of WIF-1gene in PTC tissues promoted the accumulation ofβ-catenin protein in the cytoplasm,which resulted in over activation of target gene cyclin D1.The WIF-1down regulation orβ-catenin and cyclin D1 up regulation mechanism may play an auxo-action role in the occurrence and metastasis in PTC.更多还原