【摘要】 目的探讨精子DNA完整性与复发性流产的相关性。方法病例来自于2015年1月至2016年6月在我院流产门诊就诊的夫妇,选取复发性流产患者丈夫作为研究组(n=37)和正常生育男性作为对照组(n=30)。取两组精液进行常规分析,同时采用精子染色质结构分析法(SCSA)对研究对象精子DNA完整性进行检测。结果复发性流产患者丈夫组与正常生育男性组精液的量[(3.61±0.8)mL vs(3.74±0.7)mL]、精子浓度[(130.86±69.47)×10~6/mL vs(141.97±80.44)×10~6/mL]、精子活力[(54.18±11.75)%vs(56.33±11.78)%]以及正常形态精子百分率[(13.19±8.93)%vs(14.27±8.78)%]比较差异均无统计学意义(P>0.05);复发性流产患者丈夫组精子DNA碎片指数为(28.33±7.97)%,低于正常生育男性组的(12.73±5.07)%,差异有显著统计学意义(P<0.01)。结论精子DNA完整性损伤可能是复发性流产的相关因素,SCSA对评估生育力具有临床意义。更多还原

【Abstract】 Objective To investigate the correlation between sperm DNA integrity and recurrent spontaneous abortions(RSA). Methods A total of 67 pairs of couple, who admitted to our abortion clinic from January 2015 to June2016, were selected as the research subjects. The husbands of RSA patients(n=37) were enrolled as the research group,and the husbands of healthy fertility women(n=30) were taken as the control group. The sperm of the two groups were analyzed by conventional method, and at the same time sperm DNA integrity was identified by sperm chromatin structure analysis(SCSA). Results The semen volume, sperm concentration, sperm vigor and normal sperm morphology percent of the research group were(3.61±0.8) mL,(130.86±69.47)×10~6/mL,(54.18±11.75)%,(13.19±8.93)%, respectively, which showed no significant difference with(3.74 ± 0.7) mL,(141.97 ± 80.44) × 10~6/mL,(56.33 ± 11.78)%,(14.27 ±8.78)% of the control group(P>0.05). The DNA fractionation index(DFI) of the research group was(28.33 ± 7.97)%,which was significantly lower than(12.73 ± 5.07)% of the control group(P<0.01). Conclusion The damage of sperm DNA integrity may be associated with RSA. SCSA has a certain clinical significance in assessing fertility.更多还原