【摘要】 目的探究牛蒡根水提取物对高血压患者血清致伤血管内皮细胞Toll样受体-核转录因子(TLR-NF-κB)信号转导通路的影响。方法选取2014年12月至2016年1月于桂林医学院附属医院诊治的高血压病患者40例纳入高血压组,选择健康体检者40例纳入对照组。分别采用两组研究对象的血清干预人脐静脉内皮细胞(HUVEC-C)24 h,应用不同浓度的牛蒡根水提取物干预高血压患者血清诱导的HUVEC-C TLR4表达(24 h)及核转录因子(NF-κB)活化(2 h)。将细胞连续培养24 h后将细胞蛋白提取,并用免疫印迹(Western blot)技术检测细胞内TLR4蛋白及NF-κB抑制因子I-κBα蛋白的表达。结果 1高血压组细胞提取蛋白中的TLR-4水平为(0.670 1±0.091 18),明显高于对照组(0.224±0.030 97),I-κBα水平为(0.291 5±0.054 92),明显低于对照组的(0.543 7±0.089 97),差异均有统计学意义(P<0.05);2牛蒡根水提取物干预低剂量组、中剂量组及高剂量组细胞蛋白中的TLR-4表达水平分别为(0.270 9±0.061 39)、(0.455 1±0.035 12)、(0.428 7±0.045 89),均低于高血压组的(0.670 1±0.091 18),同时高于对照组的(0.224±0.030 97);I-κBα表达水平为(0.4795±0.057 43)、(0.360 85±0.060 85)、(0.310 00±0.049 10),高于高血压组的(0.291 5±0.054 92),同时低于对照组的(0.543 7±0.089 97),差异均有统计学意义(P<0.05)。结论经TLR-NF-κB信号途径介导的免疫紊乱和炎症反应可能是高血压病血管内皮损伤的机制之一,牛蒡根水提取物可通过抑制细胞TLR-4、NF-κB的表达,从而抑制炎症因子的表达来达到保护血管内皮细胞的作用。更多还原

【Abstract】 Objective To explore the effect of burdock root aqueous extract on toll-like receptor 4-nuclear factor κ B(TLR-NF-κ B) signal pathway in vascular endothelial cells in patients with hypertension.Methods From December 2014 to January 2016,we enrolled 40 patients of essential hypertension in the Affiliated Hospital of Guilin Medical College(the hypertension group) and 40 cases healthy individuals(the control group).Human umbilical vein endothelial cells(HUVEC-C) were induced with serum collected from the two groups of subjects for 24 h.Different doses of burdock root aqueous extract were used to intervene TLR4 expression(24 h) and activation(2 h) in HUVEC-C induced by serum from patients with hypertension. The cells were continuously cultured for 24 h,and then the protein was extracted.The expression of TLR4 protein and NF-κB inhibitor I-κBα was detected by Western blot. Results(1) The TLR-4 level in the hypertension group was significantly higher than that in the control group,(0.670 1±0.0911 8) vs(0.224±0.030 97),while I-κBα level in the hypertension group was significantly lower,(0.291 5±0.054 92) vs(0.543 7± 0.089 97),with statistically significant differences(P<0.05).(2) The TLR-4 levels in the intervention groups with low-dose,middle-dose,and high-dose burdock root aqueous extract were(0.270 9±0.061 3),(0.455 1±0.035 1),(0.428 7±0.045 89),which were significantly lower than(0.670 1±0.0911 8) in hypertension group and significantly higher than(0.224±0.030 97) in the control group.(3) The I-κBα levels in the intervention groups with low-dose,middle-dose,and high-dose burdock root aqueous extract were(0.479 5±0.057 43),(0.360 85±0.060 85),(0.310 00±0.049 10),respectively,which were significantly higher than(0.291 5±0.054 92) in the hypertension group and significantly lower than(0.543 7±0.089 97) in the control group. The above differences were all statistically significant(P<0.05). Conclusion Immune disorders and inflammatory response mediated by TLR-NF-κB signal pathways may be one of the mechanisms of vascular endothelial injury in hypertensive disease. Burdock root aqueous extract can inhibit the expression of TLR-4,NF-κB,thereby inhibiting the expression of inflammatory cytokines to protect vascular endothelial cells.更多还原