Lonicera japonica, obtained from Shandong and Guangdong, was used as the raw material in this study. The volatile oil was separated by CO2 supercritical fluid extraction, and afterwards the water-soluble and the water-insoluble subfraction were prepared from the residue after volatile oil isolation. The chemical composition of volatile oil was analyzed by GC-MS and high performance liquid chromatography was used to determine the content of chlorogenic acid and galuteolin in residue extract of Lonicera japon...