The effects of the mass ratio of chloroacetic acid to schizophyllan, reaction time, and reaction temperature on the degree of substitution in carboxymethyl schizophyllan were studied using response surface experiments. The in vitro weight method and body surface instrument method were used to study the moisturizing effects of carboxymethyl schizophyllan, which were then compared with the commonly used cosmetic moisturizers glycerol(Gl) and hyaluronic acid(HA). The response surface experiments showed that, u...