【摘要】 从红豆杉树皮中分离得到内生真菌,液体培养基培养后提取其发酵产物,然后以紫杉醇和巴卡亭Ⅲ为标准品,用薄层色谱(TLC)、高效液相色谱(HPLC)等技术检测,筛选产紫杉醇或产紫杉烷类化合物的内生真菌。结果筛选出一株产巴卡亭Ⅲ的内生真菌12.4.1,其发酵产物与巴卡亭Ⅲ对照品有相近的TLC迁移斑点,且HPLC中,在巴卡亭Ⅲ对照品色谱峰相近保留时间处有吸收峰;显微鉴别表明菌株12.4.1属于曲霉菌。更多还原

【Abstract】 Endophytic fungi were isolated from Taxus by aseptic techniques and then incubated in liquid culture,its fermentation was obtained.The taxol-producing fungus was identified by TLC and HPLC compared with taxol and baccatinⅢ standard.Results showed that one strain of fungus 12.4.1 could produce baccatin Ⅲ,its fermentation and baccatin Ⅲhad the same retention time in TLC and HPLC,and fungus 12.4.1 belonged to Aspergillus according to its morphology.更多还原