【摘要】 目的:观察丹皮酚对单纯疱疹病毒性脑炎（HSE）小鼠肿瘤坏死因子-α（TNF-α）、白介素-6（IL-6）表达的影响。方法:采用颅内注射法复制HSE动物模型,将BABL/c小鼠随机分为对照组和HSV-1感染组,分别于颅内注入20μL DMEM培养基和HSV-1病毒液(LD50,10^-4/20μL),并于造模后第1天起开始给予等体积丹皮酚(30 mg/kg·d^-1)或生理盐水灌胃;经不同处理后,分为DMEM+盐水组、DMEM+丹皮酚组、HSV-1+盐水组、HSV-1+丹皮酚组,每组10只小鼠,观察各组小鼠发病情况,并在第5天断头取右侧颞叶脑组织,用酶联免疫吸附法（ELISA）测定TNF-α、IL-6的含量。结果:与对照组比较,HSV-1感染组小鼠脑组织TNF-α、IL-6含量表达明显升高（P<0.05）;丹皮酚干预后TNF-α和IL-6表达均明显降低（P<0.05）。结论:丹皮酚对HSE具有一定的抗炎作用。更多还原

【Abstract】 Objective: To observe the effect of paeonol on the expression of tumor necrosis factor（TNF）-α and interleukin（IL）-6 in herpes simplex encephalitis（HSE）mice. Methods: BABL/c mice were randomly divided into two groups: the control group in which the mice were intracranially injected with 20 μL DMEM,and the HSV-1 group in which the mice were intracranially injected with HSV-1 virus solution(LD50,10^-4/20 μL).Subsequently,the mice in the control group were sub-divided into DMEM + normal saline（NS）group and DMEM+paeonol group,and those in the HSV-1 group were sub-divided into HSV-1+NS group and HSV-1+ paeonol group（n=10 per group）. The levels of TNF-α and IL-6 in right temporal brain tissues were measured by enzyme-linked immunosorbent assay（ELISA）. Results: The expression levels of TNF-α and IL-6 in HSV-1 group were significantly higher than those in the control group（P<0. 05）. However,the levels of TNF-α and IL-6 were markedly decreased after paeonol intervention（P<0. 05）. Conclusion: Paeonol had anti-inflammatory effect on HSE in mice.更多还原