【摘要】 目的:探讨急性移植物抗宿主病(aGVHD)患者miR-155的表达水平及其临床意义。方法:采用造血干细胞移植患者移植第0、第7、第14、第21及第28天的血浆标本,实时荧光定量PCR(qPCR)方法检测miR-155相对表达量,酶联免疫吸附试验(ELISA)法检测患者血浆中白细胞介素(IL)-4、IL-7、IL-10的含量。结果:miR-155在移植后第7天开始逐渐升高,到第28天时升高明显,患者移植后不同时间点miR-155相对表达量比较差异具有统计学意义(P<0.05)。allo-HSCT后不同时间点IL-4、IL-7、IL-10水平比较,差异均无统计学意义(P>0.05)。结论:allo-HSCT后miR-155较IL-4、IL-7、IL-10水平变化出现早,可作为早期诊断和预测a GVHD的无创性生物标记物。更多还原

【Abstract】 Objective: To evaluate the expression and clinical significance of mi R-155 in patients with acute graft-versus-host disease(a GVHD). Methods: mi RNA was extracted from the plasma samples of patients day0,day 7,day 14,day 21,day 28 after their hematopoietic stem cell transplantation(HSCT). The expressions of mi R-155,interleukin(IL)-4,IL-7 and IL-10 were detected by q PCR. Results: The expression of mi R-155 obviously increased on day 7 after HSCT,and reached the peak on day 28. The mi R-155 expression level was increased with the prolonged time after transplantation(P<0. 05). There was no significant difference in the expression of IL-4,IL-7 and IL-10 between any time points(P>0. 05). Conclusion: Mi R-155 might be a noninvasion biomarker for early detection of a GVHD,and the level of mi R-155 changed earlier than l L-4,IL-10 and IL-7 after HSCT.更多还原