【摘要】 目的:研究人脐静脉内皮细胞(HUVEC)对T24膀胱癌细胞侵袭、迁移及增殖能力的影响。方法:构建HUVEC和T24膀胱癌细胞的Trans-well共培养模型(共培养组),与T24膀胱癌细胞非共培养组进行比较,分别应用Trans-well小室、Matrigel胶及CCK-8法比较两组细胞的侵袭、迁移及增殖能力。结果:侵袭实验24h后,共培养组与非共培养组的侵袭细胞数目分别为(93.22±27.35)个、(52.04±22.54)个,共培养组侵袭细胞数显著高于非共培养组(P<0.01)。迁移实验24h后,共培养组与非共培养组的迁移细胞数目分别为(183.62±62.07)个、(66.80±23.36)个,共培养组迁移细胞数显著高于非共培养组(P<0.01)。在增殖实验中,共培养组和非共培养组的吸光度(OD)值在第3、第4、第5天分别为(1.128±0.125)、(0.866±0.068)、(1.536±0.098)和(1.103±0.153)、(1.910±0.139)、(1.550±0.177)。共培养组的细胞增殖率显著高于非共培养组(P<0.01)。结论:在三维共培养条件下,HUVEC对T24膀胱癌细胞的侵袭、迁移及增殖能力均有促进作用。更多还原

【Abstract】 Objective:To assess the effects of co-culturing human umbilical vein endothelial cells(HUVEC)with T24 bladder cancer cells on the invasion,migration and proliferation of T24 cells.Methods:We divided the study into two groups:a T24 bladder cancer cell group(T24group)and the HUVEC-T24 bladder cancer cells trans-well co-culture group(co-culture group).The cell invasion,migration ability,and proliferation were compared using trans-well cell invasion Matrigel assay,transwell migration assay and CCK-8proliferation assay.Results:After 24 hours,the number of invasive and migrated cells in the co-culture group were respectively93.22±27.35 and 183.62±62.07,which were significantly higher than those in the T24group(52.04±22.54 and 66.80±23.36)(P <0.01).The CCK-8assay showed that the OD values on day 3,4and 5were respectively(1.128±0.125),(0.866±0.068)and(1.536±0.098)in the co-culture group,and were(1.103±0.153),(1.910±0.139)and(1.550±0.177)in the T24 group.The cell proliferation rate was markedly higher in the co-culture group than the T24group(P <0.01).Conclusion:Co-culture of HUVEC with T24 bladder cancer cells promotes the invasion,migration and proliferation of T24 cells.更多还原