【Objective】The optimal medium for Bletilla striata(Thumb.)Reichb.f.protocorm induction and proliferation were screened and effective protocorm proliferation system of B.striata was establishand to provide technical support for industrialized production of tissue culture seedlings of B.striata.【Method】B.striata seeds were used as explant to select the proper methods of aspetic germination.Taking MS or 1/2 MS as the basic medium,and adding plant growth substance with different concentrations and additives to ...