Two-component systems(TCS) are commonly used for transmembrane signal transduction in bacteria,They play an important role in the adaptation mechanisms between bacteria and the environment.The genus Cupriavidus is a typical heavy metal-resistant bacterium,having resistance to various heavy metals.However,there were few studies of TCS in Cupriavidus yet.Based on the results of previous researches and the method of genome-wide bioinformatics analysis,this study used Cupriavidus gilardii CR3 as type strain,ide...