【摘要】 引入多壁碳纳米管(multi-walled carbon nanotubes,MWCNTs)对壳聚糖/明胶/透明质酸(chitosan/gelatin/hyaluronic acid,Cs/Gel/HA)复合支架性能进行优化。采用冷冻干燥法制备出MWCNTs含量为2%、6%以及10%的Cs/Gel/HA/MWCNTs导电支架,对支架的理化性能及生物相容性进行评价。导电支架孔径为90~130μm,孔隙率为83%~96%;扫描电镜(SEM)及接触角测量结果显示,MWCNTs增加了孔壁粗糙度,提高了支架疏水性;支架电导率随MWCNTs含量的增加而增大,最大可增加至对照组的133.82%;MWCNTs含量为6%的支架细胞活力可增加至对照组的111.07%,且具有更高的细胞粘附率。研究初步表明,制备的Cs/Gel/HA/MWCNTs导电支架具有更好的生物相容性,可进一步应用于神经组织工程研究。更多还原

【Abstract】 Multi-walled carbon nanotubes(MWCNTs) were chosen to optimize the performance of Cs/Gel/HA composed scaffolds. Cs/Gel/HA/MWCNTs electrical conductive scaffolds with MWCNTs contents of 2%, 6% and 10% were prepared by freeze-drying. The physical and chemical properties together with biocompatibility of the scaffolds were evaluated. The pore size of the scaffolds is 90~130 μm with porosity of 83%~96%. MWCNTs can increase pore wall roughness and enhance scaffold hydrophobicity. The electrical conductivity of the scaffolds increases with the increase of MWCNTs contents, which can reach to 133.82% of that of the control. Cell viability of the scaffold with MWCNTs content of 6% can be increased to 111.07% of that of the control and cell adhesion rate increases too. Preliminary study shows that the Cs/Gel/HA/MWCNTs electrical conductive scaffolds have better biocompatibility and can be further applied in nerve tissue engineering.更多还原