【摘要】 采用微藻培养来降低空气中二氧化碳含量是当前研究的热点。然而,以气态CO₂为碳源的微藻培养过程中,普遍存在传质和固碳速率较低等问题。研究发现有机胺类物质如MEA（乙醇胺）、DEA（二乙醇胺）、MDEA（N-甲基-二乙醇胺）、TEA（三乙醇胺）等能够显著提高CO₂的气液传质速率。而针对有机胺类物质影响微藻培养及固碳效率的公开报道却并不多。研究以钝顶螺旋藻（FACHB-901）利用低含量CO₂气体为碳源的培养过程为对象,系统研究了有机胺类物质的种类（MDEA、MEA、DEA、TEA）及其添加策略对螺旋藻生物量和固碳速率的影响关系。研究结果表明,在改良的Zarrouk培养基中添加了1 mmol·L^-1 MDEA可以使培养基中溶解性无机碳（DIC）含量达到297.4 mg·L^-1。而添加1mmol·L^-1 TEA可以得到最佳的螺旋藻生物量(0.894 g·L^-1)和固碳速率(139.3 mg·（L·d）^-1)。同时,研究获得螺旋藻培养过程中TEA的最优添加策略为:延滞期添加5 mmol·L^-1、对数前期补加1 mmol·L^-1、对数后期补加2 mmol·L^-1。最终,通过TEA优化的添加策略获得了螺旋藻的生物量(1.248 g·L^-1)和固碳速率(191.4 mg·（L·d）^-1,比对照组分别提高了25.6%和41.2%。更多还原

【Abstract】 Research on biological CO₂ fixation with aquatic and marine microalgae has attracted great attention. However, when CO₂（g） is the only carbon source in microalgae cultivation, there are problems such as low CO₂ mass transfer and carbon fixation rates. Research found that alkanolamine substances, such as MEA（an ethanol amine）, DEA（diethanolamine）, MDEA（N-methyl diethanolamine） and TEA（triethanolamine） significantly increase gas-liquid mass transfer rates of CO₂ into aqueous solution. However, three are few studies discussed effects of alkanolamine on microalgae cultivation and CO₂ fixation efficiency. This study investigated Spirulina platensis（FACHB-901） cultivation processes with low CO₂（g） loading gas as the only carbon source. Effects of alkanolamine type（MDEA, MEA, DEA, and TEA） and alkanolamine adding strategy on biomass production and carbon fixation rates of Spirulina platensis were systematically studied. The results show that the soluble inorganic carbon（DIC） in the modified Zarrouk medium is 297.4 mg×L^-1 with the addition of 1 mmol×L^-1 MDEA, and the optimal biomass production(0.894 g×L^-1) and carbon fixation rate 139.3 mg×（L×d）^-1 are obtained via addition of 1 mmol×L^-1 TEA. Meanwhile, the optimal TEA adding strategy during the whole cultivation process is also obtained experimentally, which is 5 mmol×L^-1 TEA addition during delay phase, 1 mmol×L^-1 TEA at the earlier stage of logarithmic phase and 2 mmol×L^-1 TEA at the later stage of logarithmic phase. The final biomass amount of 1.248 g×L^-1 and carbon fixation rate of 191.4 mg×（L×d）^-1 are achieved, which are 25.6% and 41.2% higher than those of the control, respectively.更多还原