【摘要】 目的本研究拟运用化学计量学方法研究抑郁症肝郁气滞证关联的血清指标的变化特征。方法收集临床诊断为抑郁症肝郁气滞证患者51例,随机招募年龄、性别和教育程度相匹配的健康对照组22例,测定血清5-HT、VIP、CREB、NA和NO水平,采用t检验结合化学计量学方法同时分析其含量变化特征。结果 t检验发现抑郁症肝郁气滞证患者血清5-HT、CREB、VIP水平显著低于正常人(P<0.05)。化学计量学方法可区分两组,并对血清关联指标进行重要性排序:5-HT>CREB>1>VIP>NA>NO。结论本研究通过化学计量学分析方法发现5-HT与CREB可作为抑郁症肝郁气滞证患者的潜在标志物,为辨别该证型奠定了方法学基础,为进一步研究中医证候关联生物客观指标提供新的分析手段。更多还原

【Abstract】 Objective The present study aimed to explore serum objective indicators related to depression patients with liver Qi stagnation syndrome by using chemometrics method. Methods The present study enrolled 51 depression patients with liver Qi stagnation syndrome and 22 age-,gender-and education-matched healthy controls. Serum parameters(5-HT,VIP,CREB,NA,NO)were measured by ELISA. Furthermore,t-test and chemometrics method were performed on the data analysis. Results The levels of 5-HT,CREB and VIP of patients were significantly lower compared with healthy controls. The chemometrics method showed that the patients could be discriminated from healthy controls. Chemometrics method can rank these indicators according to their importance. :5-HT>CREB>1>VIP>NA>NO. Conclusion Our study found that 5-HT and CREB could be used as potential biomarkers of depression patients with liver and qi stagnation syndrome,laid a foundation of methodology of TCM syndrome differentiation in the syndrome,and provided a better analysis method for further study on objective indicators related to TCM syndrome.更多还原