【摘要】 本研究旨在通过高温处理和常规厌氧菌分离法从健康肥猪粪便中分离芽孢杆菌,并通过培养性状、生化试验、PCR、肠道耐受、抗菌活性和接种试验动物等方法鉴定筛选性状稳定、优良的益生性芽孢杆菌。结果显示,共分离到6株芽孢杆菌,其中1株巨大芽孢杆菌、1株死谷芽孢杆菌、2株解淀粉芽孢杆菌和2株枯草芽孢杆菌;分离菌对多黏菌素和青霉素耐药性较强,对其他12种常用药物较敏感,对致病性大肠杆菌和金黄色葡萄球菌的抑菌效果较好;4种芽孢杆菌均可在酸性、高胆盐和高温条件下生存,特别是巨大芽孢杆菌和枯草芽孢杆菌耐受能力较强;分离的芽孢杆菌对小鼠均无致病性,其中巨大芽孢杆菌和死谷芽孢杆菌有较明显的促进试验动物生长的作用。结果提示,巨大芽孢杆菌和死谷芽孢杆菌可作为益生性芽孢杆菌的初步选择。更多还原

【Abstract】 This study was aimed to separate Bacillus from healthy pig feces by high temperature treatment method and conventional anaerobic bacteria isolated method,and through the cultivation of traits,biochemical tests,PCR,bowel tolerance,antibacterial activity and vaccinated animals and other screening methods to identify stable properties and probiotic Bacillus.As a result,a total of six strains of Bacillus,which were one strain of huge Bacillus,one strain of Death Valley Bacillus,two strains of Bacillus amyloliquefaciens and two strains of Bacillus subtilis.Isolates were resistant to polymyxin and penicillin,were more sensitive to the other 12 kinds of commonly used drugs,and had better bacteriostatic action to pathogenic E.coli and Staphylococcus aureus.Four kinds of Bacillus were available under acidic,high temperature conditions and bile salts,in particular Bacillus megateriumand Bacillus subtilis were strong tolerance.Those isolated Bacillus were not pathogenic to mice,wherein Bacillus megateriumand Death Valley Bacillus had a more significant role in promoting the growth of animals.Finalized,the Bacillus megaterium and the Death Valley Bacillus could initially be as probiotic Bacillus choice.更多还原