【摘要】 目的探讨microRNA-218在肝细胞癌(HCC)中的表达水平及功能。方法选取该院肝胆外科收治的46例HCC手术患者,并将其分为转染组和未转染组,比较两组HepG2细胞的microRNA-218表达、增殖、凋亡情况,以及B细胞特异性莫洛尼白血病病毒插入位点1(Bmi-1)和周期蛋白依赖性激酶6(CDK6)的表达水平。结果肝癌组织中microRNA-218表达水平明显低于癌旁组织(P<0.05);microRNA218的表达水平与HCC的肿瘤大小、肿瘤TNM分期等临床病理特征密切相关(P<0.05);转染组HepG2细胞增殖率在转染后24、48、72h均明显低于未转染组(P<0.05);转染组HepG2细胞凋亡率明显高于未转染组(P<0.05);HepG2细胞转染后的Bim-1、CDK6表达水平均明显低于未转染组(P<0.05)。结论 microRNA-218可通过下调潜在靶点的Bim-1、CDK6表达水平来抑制肝癌细胞增殖并促进其凋亡。更多还原

【Abstract】 Objective To study the expression level and function of micro RNA(microRNA)-218 in hepatocellular carcinoma(HCC).Methods 46 cases of HCC surgery in the hepatobiliary surgery department of this hospital were selected and divided into the transfection group and non-transfection group.The expression,proliferation and apoptosis of microRNA-218 and the expression level of B cell specific Maloney leukemia virus insertion site 1(Bmi-1)and cycling-dependented kinase 6(CDK6)in HepG2 cells were compared between the two groups.Results The expression level of microRNA-218 in HCC tissue was significantly lower than that in paracancerous tissues(P<0.05);the microRNA218 expression level was closely correlated with the clinicopathological characteristics such as tumor size and TNM stage(P<0.05);the HepG2 cell proliferation rates at 24,48,72 hafter transfection in the transfection group were significantly lower than those in the non-transfection group(P<0.05);the HepG2 cell apoptosis rate in the transfection group was significantly higher than that in the non-transfection group(P<0.05);the Bim-1 and CDK6 expression levels after HepG2 cell transfection in the transfection group were significantly lower than those in non-transfection group(P<0.05).Conclusion microRNA-218 can suppress the proliferation of HCC cells and promotes HCC cells apoptosis by down-regulating the Bim-1 and CDK6 expression level in potential targets.更多还原