【摘要】 目的探讨丹参酮ⅡA(TanⅡA)抑制血小板活化的G蛋白信号途径机制。方法采用噻唑蓝(MTT)实验确定凝血酶和TanⅡA对血小板的最适作用终浓度和最适作用时间。分别采用核酸印迹法(Northern blot)和蛋白印迹法(Western blot)检测未处理组(对照组)、凝血酶处理组和TanⅡ处理组G蛋白及相关信号分子蛋白酶激活受体(PARs)、P2Y1、P2Y12、α2A-肾上腺素能受体、血栓烷A(TXA2)受体及血栓烷A2(TXA2)受体基因转录水平及蛋白表达水平,同时检测血小板聚集率。结果凝血酶处理组血小板聚集率、G蛋白及相关信号分子基因转录水平和蛋白表达水平高于对照组(P<0.05)。不同浓度TanⅡA处理组G蛋白及相关信号分子基因转录水平及蛋白表达水平明显低于凝血酶处理组(P<0.05)。结论 TanⅡA可通过抑制G蛋白及相关信号分子的基因转录和表达抑制血小板活化。更多还原

【Abstract】 Objective To discuss the mechanism of inhibition of platelet activation by tanshinone typeⅡA(TanⅡA)through G protein signal pathway.Methods Methylthiazolyldiphenyl-tetrazolium bromide(MTT)test was used to determine the optimum effective concentration and optimal time of thrombin and TanⅡA on platelet.Northern blot and Western blot were used to detect the transcription and expression levels of G protein and related signal molecules,including protease activated receptors(PARs),P2Y1 and P2Y12receptors,α2A-adrenergic receptor and thromboxane A2(TXA2)receptor,in control group,thrombin treated group and TanⅡA treated group,and the platelet aggregation rate was also detected.Results Platelet aggregation rate,and the transcription and expression levels of G protein and related molecules in thrombin treated group were higher than control group(P<0.05).The transcription and expression levels of G protein and related molecules in different concentrations of TanⅡA treated groups were lower than thrombin treated group(P<0.05).Conclusion TanⅡA could inhibit the activation of platelet by inhibiting the transcription and expression of G protein and the related molecules.更多还原