【摘要】 目的分析丙型肝炎病毒(HCV)RNA分型试剂盒检测深圳市抗-HCV阳性献血者结果。方法收集2014-2015年158份深圳市抗-HCV阳性献血者血液样本,应用聚合酶链反应(PCR)-荧光探针法进行HCV RNA定量检测,病毒载量>1.0×103 IU/mL的样本经HCV RNA分型试剂盒检测HCV基因型,分析不同基因型所占比例,病毒基因型与载量之间的相关性。结果 158份抗-HCV阳性献血者PCR-荧光探针法检出HCV RNA阳性样本54例,病毒载量>1.0×103 IU/mL的45例,全部得到分型结果,HCV 1b型、2型、3型、6型分别占57.78%(26/45)、6.67%(3/45)、8.89%(4/45)、26.67%(12/45)。单因素方差分析结果显示,1b型与2型病毒载量差异有统计学意义(F=2.861,P<0.05);不同性别间HCV RNA定量检测结果及抗-HCV S/CO值结果差异有统计学意义(P<0.05);不同年龄段各基因型分布比例经Fisher精确检验,差异有统计学意义(P<0.05)。结论 HCV 1b型、6型仍为深圳市无偿献血人群感染HCV的主要基因型,而HCV 2型和3型比例有所减少。更多还原

【Abstract】 Objective To evaluated the HCV genotyping results which obtained by genotype diagnostic kit in Shenzhen area.Methods 158 samples which ELISA test of anti-HCV were positive were collected from voluntary blood donors from 2014to2015,and were tested by PCR fluorescence probe method for viral load.The samples which viral load were greater than 1.0×103IU/mL were then tested by HCV RNA genotype diagnostic kit.To analysis the proportion of different genotypes and the correlation between genotypes with vrial load.Results 54 HCV RNA reactive sample were quantity by PCR fluorescence probe method from 158anti-HCV positive samples.The genotyping data for 45 cases which vrial load greater than 1.0×103 IU/mL were obtained by HCV RNA genotype diagnostic kit.The frequencies HCV genotype 1b,2,3and 6were 57.78%(26/45),6.67%(3/45),8.89%(4/45)and 26.67%(12/45),respectively.One-way ANOVA analysis showed that significant difference in viral loads was found between different HCV genotype 1band 2(F=2.861,P<0.05),and there was a significant difference in viral loads and anti-HCV S/CO by sex(P<0.05).Fisher′s exact test showed the significance difference between age and genotypes(P<0.05).ConclusionHCV 1band 6were the most predominant genotypes due to the higher viral load than the other subtypes among volunteer blood donors in Shenzhen,while the proportion of HCV 2,3declined.更多还原