【摘要】 目的探讨肺炎支原体(Mp)对红霉素耐药与耐药基因之间的关系。方法通过对46株红霉素耐药Mp临床分离株的23SrRNAⅤ区进行聚合酶链反应(PCR)扩增,并将扩增产物进行基因测序,确定其基因型。结果 46株红霉素耐药Mp临床分离株中有44株(95.65%)发生A2063G突变,2株(4.35%)A2064G突变,未见A2063C和C2617G/A位点突变。结论 Mp临床分离株对红霉素耐药与23SrRNAⅤ区基因2063、2064位点A→G突变有密切关系,其中最主要的突变是A2063G突变。快速、准确识别Mp的23SrRNAⅤ区基因突变可在诊断的同时给出耐药信息,有效指导临床合理用药。更多还原

【Abstract】 Objective To study the relationship between erythromycin sensitivity and drug-resistance gene in Mycoplasma pneumoniae(Mp).Methods In 46erythromycin-resistant MP clinical isolates,domain Ⅴ of 23 SrRNA was amplified by polymerase chain reaction(PCR),followed by direct automatic sequencing method.The DNA sequences were compared to find molecular mechanisms of drug resistance.Results Among the 46erythromycin-resistant Mp clinical isolates,44(95.65%)harbored an A-to-G transition mutation at position 2063 in the 23 SrRNA gene and 2(4.35%)harbored an A-to-G transition mutation at position 2064,but no A-to-C transition mutation at position 2063 and C-to-G/A transition mutation at position 2617 were detected.Conclusion Erythromycin-resistant of Mp clinical isolates were closely related to A-to-G transition mutation at position 2063/2064 in domainⅤof 23 SrRNA genes and the most important was the A2063 Gtransition mutation.Rapid and accurate identification of the genetic mutations in domain Ⅴ of 23 SrRNA may be help to diagnose the infection of Mycoplasma pneumoniae,provide the drug-resistant information,and instruct the application of antibiotics reasonably and effectively.更多还原