【摘要】 反硝化滤池是污水处理厂强化脱氮的重要技术途径之一。研究了进水碳氮比（COD/N）和空床停留时间（EBRT）对某再生水厂后置反硝化滤池反硝化脱氮性能的影响,同时考察了生物膜特征及其活性。在进水COD/N值为4.5、EBRT为28.2 min时,NO₃^--N去除率最高为72.69%,去除负荷为0.42 g N/（m²·d）;在相近EBRT（27.7 min）下,提高进水COD/N值至6.5和7.5,NO₃^--N去除负荷明显提高,分别为0.75和0.73 g N/（m²·d）。在反硝化滤池底部滤层NO₃^--N的沿程降解符合半阶动力学方程,半阶动力学系数随EBRT的降低和进水COD/N值的提高而增大。反硝化所需COD/N值平均为5.67。中层滤料生物膜的生物量及其厚度最大（分别为8 678.43 mg/m²和131.25μm）。生物膜密度由下至上逐渐增大,分别为52.58、66.12和104.59mg/cm³。反硝化活性由下至上略微增大,分别为11.34、11.44和13.47 mg N/（g VSS·h）。生物膜微生物以Beta变形菌为主,相对丰度大于85%;Methylophilaceae科菌群相对丰度最高（42.1%）;所检测到的主要菌属包括Georgfuchsia（24.3%）和Sulfuricella（4.6%）。更多还原

【Abstract】 Denitrifying biofilter is one type of the enhanced nitrogen removal technology.The nitrogen removal efficiency of a full-scale post-denitrifying biofilter(PDN)was examined under different EBRTs(empty bed retention times)and different COD/N(carbon/nitrogen)ratios.Meanwhile,the biofilm characteristics(density and thickness)and the maximum surface denitrifying rate were evaluated.Under the COD/N ratio of 4.5 and EBRT of 28.2 min,the nitrate removal rate was 72.69%,with the corresponding removal load of 0.42 g N/(m~2·d).Under similar EBRT(27.7 min),enhancing COD/N ratios to 6.5 and 7.5,the nitrate removal load was improved to 0.75 and 0.73 g N/(m~2·d),respectively.In the bottom layer of PDN,the dynamics of nitrate removal fitted well with the half-order reaction,and the half-order coefficient increased with decreasing EBRTs and increasing COD/N ratios.The required COD/N ratio for denitrification was averagely 5.67.The biofilm in middle layer of PDN possessed the highest biofilm biomass and thickness(8 678.43 mg/m~2,131.25μm).The biofilm densities in different PDN depths were 52.58,66.12 and 104.59 mg/cm~3respectively,increasing from the bottom layer to the upper layer.The maximum denitrifying rate in the bottom,middle and upper layers of PDN were 11.34,11.44 and 13.47 mg N/(g VSS·h),respectively.The dominating bacteria in biofilm was Beta-proteobacteria with the abundance over 85%.Methylophilaceae had the highest abundance(42.1%)at the family level.The main detected bacteria at the genus level were Georgfuchsia(24.3%)and Sulfuricella(4.6%).更多还原