【摘要】 以丝素蛋白(RSF)/膀胱脱细胞基质(BAM)/透明质酸(HA)水溶液为纺丝液,通过静电纺丝法制备RSF/BAM/HA复合纤维支架。通过扫描电子显微镜、ELISA、MTT以及荧光显微镜对支架的形貌和体外生物学性能进行表征。结果表明,3组分比例不变时,纺丝液浓度对纤维支架形貌影响不明显;从支架中成功检测出血管内皮生长因子(VEGF)、血小板衍化生长因子(PDGF-BB)和角化细胞生长因子(KGF)及其缓释行为曲线;这些生物活性因子使RSF/BAM/HA复合纤维支架比纯RSF支架更有利于细胞的粘附与增殖。更多还原

【Abstract】 Regenerated silk fibroin(RSF)/bladder acellular matrix(BAM)/hyaluronic acid(HA)scaffolds were electrospun from aqueous solution.The morphology and biological performances in vitro were characterized by scanning electron microscope,ELISA,MTT and fluorescent microscopy.Results showed that the concentrations of spinning solutions had limited impact on the morphology of the scaffolds.The existence,bioactivity and continuous release behaviors of vascular endothelial growth factor(VEGF),platelet-derived growth factor BB(PDGF-BB),keratinocyte growth factor(KGF)from the scaffolds were confirmed and determined.Due to the bioactivity of the growth factors,the RSF/BAM/HA scaffolds showed better performance for cell growth and proliferation than bare RSF scaffolds.更多还原