【摘要】 目的建立人尿液中8-羟基脱氧鸟苷（8-OHd G）检测方法并探讨其在肿瘤诊断中的应用价值。方法以8-羟基脱氧鸟苷为外标,尿液经离心过滤后进行分析。色谱柱:Agilent HC-C18Column（4.6 mm×250 mm,5μm）;流动相为50 mmol·L^-1Na H₂PO₄溶液（p H=3.5）-甲醇=90%∶10%,流速:1.0m L·min^-1;检测波长:300 nm;进样量:50μL。用HPLC法检测肿瘤患者尿液中8-OHd G质量浓度,对照组为健康人,试验组纳入肺癌患者手术前后、肿瘤患者化疗前后的病例。结果该方法线性关系良好;最低定量限为0.1μg·m L^-1;日内、日间精密度（RSD）均<2%;加标回收率均>90%。肺癌术前组、肺癌术后组与健康人组的8-OHd G水平分别为（5.49±5.48）,（0.51±0.63）,（1.16±1.65）μg·m L^-1。肺癌术前组的8-OHd G质量浓度显著高于肺癌术后组和健康人群组,差异有统计学意义（P<0.05）。结论用HPLC法检测尿液中的8-OHd G在肿瘤诊断和化疗疗效评估方面具有较高的准确度和特异性。更多还原

【Abstract】 Objective To develop a method for the detection of 8-hydroxy deoxyguanosine（ 8-OHd G） in urine and to explore its value in tumor-assisted diagnosis. Methods The chromatographic separation was performed on Agilent HC-C18Column（ 4. 6 mm × 250 mm,5 μm）with the mobile phase of 90% 50 mmol·L^-1Na H₂PO₄ aqueous solution and 10% methanol at a flow rate of 1. 0 m L · min^-1. The detection wavelength was 300 nm. The injection volume was 50 μL. The concentration of 8-OHd G in the urine of patients with cancer was detected by HPLC. The control group was healthy person and the patients were divided into two groups: before and after the operation,before and after chemotherapy. Results The method had a good linear relationship. The limit of detection was 0. 1 μg·m L^-1. The RSDs of inter-day and intra-day precision were less than 2%. The spiked recovery rates were more than90%. The level of 8-OHd G in lung cancer group was（ 5. 49 ± 5. 48）μg · m L^-1, the level of 8-OHd G in lung cancer group was（ 0. 51 ± 0. 63） μg·m L^-1,the level of 8-OHd G in healthy group was（ 1. 16 ± 1. 65） μg · m L^-1. The concentration of 8-OHd G in preopretive group was significantly higher than that in postoperative groupand control group（ all P < 0. 05）. Conclusion 8-OHd G detected by HPLC has high accuracy and specificity in tumor diagnosis and evaluation of chemotherapy efficacy.更多还原