【摘要】 目的研究丙泊酚全身麻醉过程对水杨酸钠排泄的影响及其机制。方法麻醉前,取雄性SD大鼠16只,腹腔注射1%水杨酸钠80 mg·kg^-1后,收集尿液,记录实际排尿时间和尿量。用光谱法测定尿液中水杨酸浓度,绘制尿药浓度-时间曲线。经3 d清洗期,上述大鼠尾静脉注射丙泊酚150 mg·kg^-1后,立即腹腔注射水杨酸钠,方法同前。绘制麻醉前与麻醉后尿药浓度-时间曲线,代入药代动力学分析软件3P97,计算尿药代动力学参数,比较麻醉前与麻醉后水杨酸钠排泄能力的变化。眶静脉丛取血检测血气指标,并进行血气指标与水杨酸累积排泄量相关分析。结果丙泊酚麻醉前与麻醉后,p H分别为7.44±0.04,7.37±0.06;二氧化碳分压（PCO2）分别为（39.58±6.42）,（47.71±6.80）mm Hg;氧分压（PO2）分别为（88.25±6.80）,（67.75±3.73）mm Hg;剩余碱（BE）分别为（1.20±1.05）,（-5.08±1.88）mmol·L^-1;最大排泄率（Rmax）分别为（0.62±0.15）,（0.39±0.04）mg·h^-1;Cmax分别为（1.43±0.23）,（0.85±0.15）mg·kg^-1;0²8 h排泄-时间曲线下面积（ERUC0-28）分别为（25.83±3.8）,（17.35±2.01）mg·h·L^-1,差异有统计学意义（均P<0.05）。p H、PO2、BE与水杨酸累积排泄量呈明显正相关,相关系数（R2）分别为0.9758,0.9760,0.9398;而PCO2与水杨酸累积排泄量呈明显反正相关,R2为0.9023,差异有统计学意义（P<0.05）。结论丙泊酚静脉麻醉能减慢大鼠水杨酸钠排泄速度,与血气指标显著相关。更多还原

【Abstract】 Objective To explore the effects of propofol intravenous anesthesia on the excretion of sodium salicylate and its mechanism in rats. Methods A total of 16 male SD rats were placed in rat metabolic cage to collect urine after intraperitoneal injection of 1% sodium salicylate 80 mg · kg^-1. Then the actual time urination and urinary volume were recorded. And salicylic acid concentration in urine were determine by spectrometry. Urine drug concentration-time curve were drawed. After 3 days of cleaning period,the rats were given intravenous injection of propofol 150 mg · kg^-1immediately after intraperitoneal injection of sodium salicylate with the former method. Urine drug concentration-time curve were drewed before and after anesthesia.Urine drug metabolism and pharmacokinetics parameters were analyzed with 3P97 Software. Sodium salicylate excretion capacity was analyzed before and after anesthesia. In experiment 3 h,the blood gas indexes were detected in the orbital venous plexus. After 3 h,blood from orbitalvenous was collected for analyzing the parameters of blood gas. And then,blood gas index and salicylic acid accumulation correlation analysis was eliminated. Results p H were 7. 44 ± 0. 04,7. 37 ± 0. 06 respectively before or after anesthesia by propofol intravenous. Partial pressure of carbon dioxide（ PCO2） were（ 39. 58 ± 6. 42）,（ 47. 71 ± 6. 80） mm Hg respectively. Partial pressure of oxygen（ PO2） were（ 88. 25 ± 6. 80）,（ 67. 75 ± 3. 73） mm Hg respectively. Base excess（ BE） were（ 1. 20 ± 1. 05）,（-5. 08 ± 1. 88） mmol · L^-1respectively. Maximum excretory rate（ Rmax） were（ 0. 62 ±0. 15）,（ 0. 39 ± 0. 04） mg· h-1respectively. Cmaxwere（ 1. 43 ± 0. 23）,（ 0. 85 ± 0. 15） mg·kg^-1respectively.Excretion under curve（ ERUC0-28） were（ 25. 83 ± 3. 8）,（ 17. 35 ± 2. 01） mg·h·L^-1respectively,the difference was statistically significant（ all P < 0. 05）. Statistically significant positive correlation was presented between p H,PO2,BE and accumulated with salicylic acid excretion（ P < 0. 05）. And R2 were respectively 0. 9758,0. 9760,0. 9398. At the same time,the obvious negative correlation was observed between PCO2 and salicylic acid accumulation excretion,R2was0. 902 3,it was statistical significance（ P < 0. 05）. Conclusion Propofol intravenous anesthesia can slow down the rate of sodium salicylate excretion,and significantly related to parameters of blood gas.更多还原