【摘要】 以罗非鱼皮为原料,通过酶的筛选和酶解正交试验,分别获得木瓜蛋白酶和碱性蛋白酶酶解罗非鱼皮的最优酶解条件,在两种酶的最优酶解条件下进行分步复合酶解制备罗非鱼皮多肽,并探究罗非鱼皮多肽对新西兰兔皮肤烫伤创面的修复作用。结果表明,木瓜蛋白酶和碱性蛋白酶的最优酶解条件分别为pH6.5、温度55℃、底物浓度30%、酶用量0.5%和pH9.5、温度45℃、底物浓度35%、酶用量0.4%,相应的蛋白质回收率为90.54(±1.72)%和89.22(±2.14)%,复合酶解所得的蛋白质回收率比最优酶解条件下的木瓜蛋白酶和碱性蛋白酶分别提高了5.03%和6.58%。烫伤愈合实验结果表明:伤口处理后17 d,实验组与阳性对照组的创面愈合率均已达到95%以上,与模型对照组相比均有显著性差异;实验组与阳性对照组的创面愈合率未见明显差异,愈合效果相近。病理组织学结果显示,实验组烫伤创面恢复情况最好,表明罗非鱼皮多肽对皮肤烫伤创面有很好的修复效果。更多还原

【Abstract】 By using tilapia skin as raw material,the optimum conditions for enzymolysis of tilapia skin with papain and alkaline protease were obtained through enzyme screening and orthogonal test of enzymolysis. The tilapia skin polypeptide was prepared by stepwise composite enzymolysis of the two enzymes under the optimal enzymolysis conditions,and the repair effect of tilapia skin polypeptide on skin scald wounds of New Zealand rabbits was investigated. The results showed that the optimum enzymolysis conditions of papain and alkaline protease were as follows: the pH,temperature,substrate concentration and enzyme dosage were 6.5 and 9.5,55℃ and 45℃,30% and 35% ,0.5% and 0.4% ,and the corresponding protein recovery rates were 90.54(±1.72)% and 89.22(±2.14)% . The protein recovery rate of composite enzymolysis increased by 5.03% and 6.58% compared to those of papain and alkaline protease under the optimal enzymolysis conditions,respectively. The results of scald healing experiment showed that the wound healing rates of the experimental group and the positive control group reached more than 95% on the 17 th day,which were significantly different from that of the model control group( P < 0.05).There was no significant difference in wound healing rate between the experimental group and the positive control group,and the healing effects were similar. Histopathological results showed that the recovery of scald wounds in experimental group was the best,indicating that tilapia skin polypeptide had a great repair effect on skin scald wounds.更多还原