【摘要】 比较了9种阳离子、碳源、氮源和pH值对烟草青枯菌生长的影响,结果表明,不同阳离子对烟草青枯菌生长的影响具有差异,在1 mmol/L浓度条件下,Ca²⁺、K⁺、Mg²⁺、Mn²⁺、Fe²⁺、Fe³⁺等离子间对青枯菌生长影响差异较小,而与Cu²⁺、Al³⁺差异较大;在10 mmol/L浓度条件下,Mn²⁺、Fe²⁺、Fe³⁺、Cu²⁺、Al³⁺等离子显著抑制青枯菌生长;在30 mmol/L浓度条件下,Ca²⁺、K⁺明显抑制青枯菌生长,而Mg²⁺在60mmol/L浓度下,仍青枯菌生长良好。此外,研究结果还表明,青枯菌生长最适宜的pH值为7,最适宜的碳源为蔗糖,最适宜的氮源为蛋白胨,硝态氮氮源不利于青枯菌生长。更多还原

【Abstract】 Tobacco bacterial wilt caused by Ralstonia solanacearum is a widespread and serious disease in China and is difficult to control. Nutrition is the material basis of microbial metabolism and all life activities. Analyzing the effects of nutrition elements on the growth of R. solanacearum is important for inhibiting the growth of pathogenic bacteria through nutrition regulation measures so as to the bacterial wilt disease control. The growth of R. solanacearum under nine kinds of cations,carbon source,nitrogen source and pH were compared in this study. The results showed that R. solanacearum growth under different cations was different. Under the concentration of 1 mmol/L,little influence of Ca²⁺,K+,Mg²⁺,Mn²⁺,Fe²⁺,Fe³⁺ were observed on R. solanacearum growth,and their effects were different from Cu²⁺ and Al³⁺. Under the conditions of 10 mmol/L of Mn²⁺,Fe²⁺,Fe³⁺,Cu²⁺ and Al³⁺ respectively,the R. solanacearum growth was inhibited significantly. When the concentration of Ca²⁺ and K+ was 30 mmol/L,the R. solanacearum growth was inhibited,while the concentration of Mg²⁺ was 60 mmol/L,R. solanacearum grew well. In addition,the results also showed that pH value 7,sucrose and peptone were the best for R. solanacearum growth. Nitrate nitrogen source was against R. solanacearum growth.更多还原