In this paper, TFP fragment was firstly obtained by PCR amplification using a plasmid which contains TFP c DNA as a template. The amplified fragment wascloned into P. Pastoris expressing vector p PIC9 K. After confirming through sequencing, a recombinant expression plasmid p PIC9K-TFPisobtained. The plasmid islinearized and then transformed into P. Pastoris cell GS115 by electroporation. The positive recombinantis screened and confirmed by PCR. A single colony is used fermented which grew at 30 ℃ in a shaki...
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