【摘要】 为探讨中药灌肠方保留灌肠治疗溃疡性结肠炎(UC)的作用机理,将40只Wister大鼠随机分为A(正常组)、B(造模组)、C(中药灌肠组)和D(西药灌肠组)4组,A组大鼠正常饲养,不予造模;B、C、D组应用三硝基苯磺酸造模法建立UC大鼠模型。造模成功后,A组和B组予生理盐水保留灌肠,C组、D组分别予以中药灌肠方、地塞米松稀释液保留灌肠。观察大鼠造模情况,并对比观察各组大鼠造模前、造模后治疗前、治疗后血清IgA和IgG含量,以及各造模组肠黏膜组织损伤情况。结果显示,各造模组均成功建立UC大鼠模型。造模后,B-D组血清IgA和IgG含量均明显升高,与A组相比,差异均有显著统计学意义,P<0.01。治疗3周后,C组和D组血清IgA和IgG含量明显降低,基本恢复正常,明显低于B组,P<0.05;但与A组比较差异无统计学意义,P>0.05。C组、D组肉眼及电镜下观察肠黏膜损伤程度评分均明显低于B组,P<0.05或P<0.01;C组与D组比较差异无统计学意义,P>0.05。结果表明,中药灌肠方保留灌肠可促进UC大鼠肠黏膜组织修复,其作用可能是通过调节机体免疫功能实现的。更多还原

【Abstract】 In order to explore the mechanism of traditional Chinese medicine enema in the treatment of ulcerative colitis(UC),40 Wister rats were randomly divided into 4 groups:group A(normal group),group B(model group),group C(traditional Chinese medicine enema group) and group D(western medicine enema group).Group A of rats were normal feed,no modeling;group B,C and D used trinitrobenzene sulfonic acid modeling method to establish UC rat model.After successful modeling,group A and group B were given saline retention enema,group C and group D were given traditional Chinese medicine enema and dexamethasone diluent retention enema respectively.The model of rats was observed.The contents of IgA and IgG in serum before and after model and treatment were compared,and the damage of intestinal mucosa tissue in each model group was observed.As results,the rats in group B,C and D were successfully established UC model.The contents of IgA and IgG in group B-D were significantly increased after modeling, compared with group A,there was statistical significance(P <0.01).After 3 weeks of treatment,the contents of serum IgA and IgG in group C and group D were significantly decreased and basically to the normal level;all were lower than those in group B( P <0.05),but compared with group A, there was no statistical significance(P >0.05).The scores of intestinal mucosal injury in group C and group D were significantly lower than those in group B,observing with eyes and electron microscope( P<0.05 or P<0.01); There was no significant difference between group C and group D( P >0.05).The results show that traditional Chinese medicine enema can promote intestinal mucosal tissue repair in UC rats,which may be through the regulation of immune function.更多还原