【摘要】 以147花椰菜和庆农65天2个花椰菜品种为试材,研究了影响花椰菜不定芽再生的各个因素,建立花椰菜高效离体再生体系。结果表明:基因型、外植体类型、激素的类型和配比等是影响花椰菜再生的主要因子。品种对再生频率影响较大,庆农65天的外植体再生频率高于147花椰菜;不同外植体再生频率也存在差异,下胚轴的再生频率显著高于子叶。147花椰菜取用7 d龄无菌苗的子叶和下胚轴,培养在MS+6-BA1 mg/L+NAA 0.2 mg/L培养基上培养效果最佳,庆农65天则培养在MS+6-BA 0.5 mg/L+NAA 0.05 mg/L培养基上效果最佳;再生芽在MS培养基,生根率达100%。本研究为花椰菜基因遗传转化构建高效的再生体系奠定基础。更多还原

【Abstract】 Two cultivars of cauliflower were investigated for factors affecting the regeneration of adventitious bud. The results indicated that the genotype, type of explants and hormones were the main factors affecting regeneration. Varieties had great influence on the regeneration frequency of explants, the regeneration frequency of Qing nong 65 were higher than 147 cauliflower; there were differences in the regeneration frequency of different explants, hypocotyl regeneration frequency was significantly higher than that of cotyledon. The explants induction rate was differentiation in different culture medium. Using cotyledons and hypocotyls of 7 d aged 147 cauliflower aseptic seedling as explants, MS medium supplemented with 6-BA 1 mg/L + NAA 0.2 mg/L was the optimum medium. However, the optimum medium of Qing nong 65 was MS medium supplemented with 6-BA 0.5 mg/L+NAA 0.05 mg/L. Inserting regeneration buds into MS medium, the rooting rate could reach 100%. This study layed a foundation for constructing efficient regeneration transformation system of cauliflower gene.更多还原