SSR markers play an important role in authenticity detection, purity identification and fingerprint library building In the process of SSR primers screening will meet imperfect SSR primers, such as N +1 peak and other abnormal peak, nonspecific peak, low polymorphism and not suitable for automatic analysis software. In order to understand and master the performance and the reasons about imperfect SSR primers, in this study, using22 materials about first screening, 27 sets of mother-father-child trios and 95...