Selection of homozygous inbred lines with the traditional breeding method requires 7~9 generations.Chromosome doubling of haploid harboring single parental chromosomes results in a doubled haploid in a single generation, which accelerates the breeding process. A few methods of haploid production and haploid doubling are constrained by species or genotype and are not applied widely. Haploid was produced by genetic modification of the centromere-specific histone 3(CENH3) in Arabidopsis thaliana. CENH3 is requ...