【摘要】 本研究利用SSR方法对46个苹果砧木资源基因组DNA进行分析,了解了目前生产上常用砧木在野生资源中的遗传背景分布,并为进一步的砧木育种亲本选择提供了参考依据。本研究选用筛选出的具有较高多态性的31对引物,获得了111个多态性位点。通过NTSYS软件进行相似性系数计算,46个苹果砧木资源的相似系数分布在0.585 6~0.964 0之间,利用UPGMA法构建聚类树状图,将供试砧木品种划分为4个类群:西府海棠单独划为一类;以山荆子系为主的7个品种为第二类;三叶海棠b、丽江山定子和平邑甜茶聚为第三类;第四类群主要是新疆地方品种、楸子类和培育的砧木品种,共32个品种,聚类又分为4个组,在第四组中大部分的新疆地方品种与楸子类互为交叉聚类在一起,而M9、PAJAM2、Luo 2和Luo 6等培育的砧木品种与花红、新疆野苹果和西蜀海棠等单独聚在一起。更多还原

【Abstract】 Genomic DNA of 46 apple rootstock resources was analyzed using SSR method, which made us knowing the genetic background distribution of common stocks in the wild resources, and provided reference for the selection of the parents of stock breeding. In this study, 31 pairs of primers with high polymorphism were selected, and 111 polymorphic loci were obtained. The similarity coefficients of 46 apple rootstocks were between0.585 6~0.964 0, which were calculated by NTSYS software. Through constructing cluster tree by UPGMA method, 46 apple rootstock resources were divided into 4 groups: M. micromalus Makino was clustered alone as the first class; 7 varieties, Ser. Baccatae mainly were clustered together as the second class; As the third class,three varieties were included: M. sieboldii(Regel.) Rehd., M. rockii Schneid. and M. hupehensis(Pamp.) Rehd.;the fourth class mainly included Xinjiang local varieties, M. prunifolia varieties and several cultivated rootstock varieties, totally 32 varieties. The fourth class was clustered and divided into 4 groups, in the fourth groups most of the local varieties of Xinjiang and M. prunifolia clustered together, while cultivated rootstock varieties such as M9, Luo2, and Luo6 clustered with M. asiatica, M. sieversii and M. prattii(Hemsl.) Schneid. together.更多还原