【摘要】 研究了两个个体特征差异较大且面对干旱有不同响应机制的木薯品种SC124和Arg7,比较了在干旱处理3 d、12 d、18 d后DNA甲基化的变化情况,探究DNA甲基化在木薯抵御干旱胁迫中的作用。利用全基因组甲基化检测技术扩增片段单核苷酸多态性位点和甲基化位点(AFSM)检测基因组上的DNA甲基化水平变化情况。根据AFSM建库标准构建了测序文库,并对文库进行了质量检测。根据AFSM测序数据分析表明,木薯基因组中叶片的甲基化比例为5.9%~28.6%,根的甲基化比例为4.1%~13.5%。干旱胁迫使植株甲基化总体水平产生变化,并且具有组织和时空特异性。木薯面对干旱胁迫根据品种的不同,DNA甲基化具有不同的相应特点,且和处理时期和组织部位相关。更多还原

【Abstract】 The objective of the study is to research the relationship between drought stress and DNA methylation in cassava by comparing the different DNA methylation level between leaves and roots in 3 d,12 d,18 d after drought stress for SC124(drought tolerance strain) and Arg7(drought sensitive strain).We use AFSM(amplifiedfragment SNP and methylation) which is a quick and simple technology based on two restriction enzyme pairs(Eco RⅠ-MspⅠ and Eco RⅠ-HpaⅡ) and a next-generation sequencing platform technology to detect the methylation variation in whole genome.We built a library based on the criterion of AFSM method and sequencing it in one line of an Illumina Hi Seq 2 500 platform with paired-end sequencing.The AFSM analysis showed that the genomicDNA methylation level of leaves in cassava were 5.9%~28.6%.The DNA methylation level of roots in cassava were 4.1%~13.5%.The genomicDNA methylation existed the variation between different tissues and changed in 3 d,12 d,18 d after drought stress.The study revealed that the pattern of DNA methylation under drought stress is temporal specific and tissue specific.Different strain had opponent response to DNA methylation under drought stress.更多还原