Objective To establish a method for extracting DNA from old bones by Auto Mate Express~(TM) system. Methods Bones were grinded into powder by freeze-mill. After extraction by Auto Mate Express~(TM),DNA were amplified and genotyped by Identifiler~Plus and Min Filer~(TM)kits. Results DNA were extracted from 10 old bone samples, which kept in different environments with the postmortem interval from 10 to 20 years, in 3 hours by Auto Mate Express~(TM)system. Complete STR typing results were obtained from 8 sam...
【基金】
福建省自然科学基金资助项目(2014J01273)
【更新日期】
2017-09-25
【分类号】
D919.4
【正文快照】
近年来,骨骼DNA检验技术已取得很大进展,在实际案件的侦破中发挥重要作用。但是,对于陈旧性骨骼,由于细胞含量少,以及长时间受埋葬环境的影响,其DNA的提取仍然是鉴定工作的难点[1]。本研究利用AutoM ate ExpressTM系统自动快速提取陈旧性骨骼DNA,并结合运用Mini FilerTM试剂盒,