【摘要】 目的调查华夏^TM白金PCR扩增试剂盒所包含的23个常染色体STR基因座在中国汉族人群中的遗传多态性,评估其在法医遗传学中的应用价值。方法应用华夏^TM白金PCR扩增试剂盒对500名汉族无关健康个体进行分型检测,统计分析所含STR基因座的频率分布及群体遗传学参数,与目前国内外常用的7种商品化试剂盒在STR基因座个数、内标、荧光标记、Ladder中等位基因总数以及系统效能上进行比较。结果 23个常染色体STR基因座均符合Hardy-Weinberg平衡（P>0.05）,个体识别率为0.791 5⁰.986 2,多态信息含量为0.559 0⁰.914 0。系统累积个体识别率高达1-4.1×10-28,三联体累积非父排除率为1-4.1×10^-10,二联体累积非父排除率为1-8.4×10^-7。通过与7种试剂盒比较,华夏^TM白金PCR扩增试剂盒含有的等位基因分型标准品（Ladder）中的等位基因数最多。结论 23个常染色体STR基因座在汉族人群中有良好的遗传多态性,可用于法医遗传学亲权鉴定及个体识别。通过与其他常见的7种商业化STR检测试剂盒比较,华夏^TM白金PCR扩增试剂盒可进一步提供更为丰富的遗传信息。更多还原

【Abstract】 Objective To investigate the genetic polymorphism of 23 autosomal STR loci of Huaxia^TM Platinum kit in Chinese Han population,and to evaluate the forensic efficiency of Huaxia^TM Platinum kit.Methods A total of 500 unrelated healthy individuals from Han population were genotyped with Huaxia^TM Platinum kit.The frequency distribution and the parameter of population genetics of STR loci were analysed statistically.Huaxia^TM Platinum kit was compared with other 7 commercial STR kits commonly seen at home and abroad in the number of STR loci,interior label,fluorescent mark,total number of alleles in Ladder and system effectiveness.Results All the 23 autosomal STR loci were consistent with Hardy-Weinberg equilibrium（P>0.05）.The discrimination power was 0.791 5-0.986 2.The polymorphism information content（PIC） was 0.559 0-0.914 0.The combined discrimination power（CDP） was 1-4.1×10-28,while combined probability of paternity exclusion in trio（CPET） and in duo（CPED） were 1-4.1×10^-10 and1-8.4×10^-7,respectively.Compared with other 7 kits,Huaxia^TM Platinum kit contained the most number of alleles within the Ladder.Conclusion All the 23 autosomal STR loci of Huaxia^TM Platinum kit with highly polymorphic in Han population can be used for paternity testing and individual identification.Compared with other 7 kits,it appears that Huaxia^TMPlatinum kit can provide more genetic information.更多还原