【摘要】 目的探究大鼠死后肌肉挥发性有机化合物(volatile organic compound,VOC)的变化规律与死亡时间(postmortem interval,PMI)的关系。方法健康大鼠120只,随机分成12组,每组10只,以颈椎脱臼方式处死后保存在(25±1)℃环境中,在死后即刻(0 d)、0.5、1、2、3、4、5、6、7、8、9、10 d共12个时间点分别取一组大鼠肌肉,采用顶空固相微萃取气质联用法收集、检测、分析大鼠肌肉VOC。结果共鉴定出15种VOC,其中芳香类9种、含硫类3种、脂肪酸类2种、杂环类1种。随PMI延长,VOC种类总数呈增加趋势,第1天没有检测到VOC,第2天检出3种,第3天检出9种,第4天检出11种,5~7 d检出14种,8~10 d检出15种。不同VOC峰面积与PMI存在一定的相关性(校准R~2=0.15~0.96),15种VOC总峰面积(y)与PMI(x)相关:2~5 d,回归方程为y=-17.05 x~2+164.36 x-246.36(校准R~2=0.96);6~10 d回归方程为y=2.24 x+101.13(校准R~2=0.97)。结论肌肉VOC变化规律有助于PMI的推断。更多还原

【Abstract】 Objective To explore the relationship between the change rules of volatile organic compounds(VOCs) in rat muscle and postmortem interval(PMI).Methods A total of 120 healthy rats were divided randomly into 12 groups(10 for each group).After the rats were sacrificed by cervical dislocation,the bodies were kept at(25±1) ℃.Rat muscle samples were separately obtained at 12 PMI points,including0,0.5,1,2,3,4,5,6,7,8,9 and 10 d.The VOCs in rat muscles were collected,detected and analyzed by headspace solid-phase microextraction(HS-SPME) coupled to gas chromatography-mass spectrometer(GC-MS).Results In total,15 species of VOCs were identified,including 9 aromatic compounds,3 sulfur compounds,2 aliphatic acids and 1 heterocyclic compound.The species of VOCs increased with PMI:no species were detected within 1 day,3 species were detected on day 2,9 on day3,11 on day 4,14 from day 5 to 7,and 15 from day 8 to 10.Total peak area of 15 species of VOCs was significantly correlated to PMI(adjusted R~2=0.15-0.96):the regression function was y=-17.05 x~2+164.36 x-246.36(adjusted R~2=0.96) from day 2 to 5,and y=2.24 x+101.13(adjusted R~2=0.97) from day6 to 10.Conclusion The change rules of VOCs in rat muscle are helpful for PMI estimation.更多还原