Determination of five alkaloids in Huanglian by high performance liquid chromatography using 1-hexyl-3-methylimidazolium tetrafluoroborate as the mobile phase additives
建立了高效液相色谱法(HPLC)同时测定黄连中5种生物碱含量的方法。黄连药材经甲醇超声提取后,用Spherigel C_(18)色谱柱(250×4.6 mm,5μm)进行HPLC测定,流动相为含有1-己基-3-甲基咪唑四氟硼酸盐添加剂的甲醇-水(25:75,V/V),流动相流速为1.0 m L/min,检测波长为345 nm,同时测定了黄连药材中药根碱、表小檗碱、黄连碱、巴马汀和小檗碱含量。在1~200μg/m L浓度范围内,5种生物碱的线性相关系数均大于0.9990,药根碱、表小檗碱、黄连碱、巴马汀和小檗碱的检测限(LOD)分别为0.19,0.13,0.11,0.18,0.15 mg/L。测定了3种不同产地的黄连生物碱的含量并进行加标回收,回收率在98%~102%之间。
【英文摘要】
A sensitive,selective method was developed for determination of alkaloids of Huanglian by high performance liquid chromatography (HPLC).Huanglian samples were extracted with methanol under sonication and analyzed by HPLC using a Spherigel C_(18) column.The mobile phases were methanol-water (25: 75,V/V) with 1-hexyl-3-methylimidazolium tetrafluoroborate as the mobile phase additives.The flow-rate of the mobile phase was 1.0 m L/min and the effluent was monitored at 345 nm.Five alkaloids including jatrorrhizi...
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