【摘要】 目的探讨COX-2基因沉默联合奥沙利铂(oxaliplatin,OXA)对胃癌细胞的杀伤作用,为胃癌治疗提供依据。方法分别采用COX-2siRNA、COX-2siRNA+OXA、OXA处理MGC803胃癌细胞株,以未经处理的MGC胃癌细胞株作为对照组,应用MTT法测定各组细胞抑制率,应用实时荧光定量PCR技术(qRT-PCR)和蛋白免疫印迹法(Western Blot)分别检测各组β-链蛋白(β-catenin)和B淋巴细胞瘤-2(Bcl-2)等基因的表达情况,并通过流式细胞术检测各组MGC803细胞凋亡情况。结果 COX-2siRNA组和COX-2siRNA+OXA组MGC803胃癌细胞抑制率和MGC803胃癌细胞凋亡率均显著高于OXA组(P<0.05),β-catenin与Bcl-2mRNA水平和蛋白水平均显著低于OXA组(P<0.05);COX-2siRNA+OXA组MGC803胃癌细胞抑制率和MGC803胃癌细胞凋亡率均显著高于COX-2siRNA组(P<0.05),β-catenin与Bcl-2mRNA水平和蛋白水平显著低于COX-2siRNA组(P<0.05)。结论 COX-2基因沉默能够降低β-catenin和Bcl-2表达,提高胃癌细胞抑制率和凋亡率,有效提高OXA药物敏感性。更多还原

【Abstract】 Objective To investigate the COX-2gene silencing combined with oxaliplatin on the gastric cancer cells,provide the basis for the treatment of gastric cancer.Methods MGC803 gastric cancer cell lines were treated with COX-2siRNA,COX-2siRNA+oxaliplatin,and oxaliplatin,respectively.MTT method was used to determine cell inhibition rate of each group,the real-time fluorescence quantitative PCR(QRT PCR)and Western blotting(Western blot)were used to detect beta chain protein(beta catenin)and B cell lymphoma 2(Bcl-2)gene expression,and flow cytometry was used to detect apoptosis of MGC803 cells situation.Results In COX-2siRNA group and COX-2siRNA+oxaliplatin group,human gastric cancer cell line MGC803 inhibition rate and gastric cancer cell line MGC803 apoptosis rate were significantly higher than those of oxaliplatin group(P<0.05),β-catenin and bcl-2mRNA level and protein level were significantly lower than those of oxaliplatin group(P<0.05).human gastric cancer cell line MGC803 inhibition rate and gastric cancer cell line MGC803 apoptosis rate in COX-2siRNA+oxaliplatin group were significantly higher than those in COX-2siRNA group(P<0.05),β-catenin and bcl-2mRNA level and protein level were significantly lower than those of COX-2siRNA group(P<0.05).Conclusion COX-2gene silencing can increase gastric cancer cell inhibition rate and apoptosis rate and reduceβ-Catenin and bcl-2expression,and effectively improve the drug sensitivity of oxaliplatin based chemotherapy.更多还原