【摘要】 目的研究氧化苦参碱对牙龈卟啉菌内毒素(Pg-LPS)作用下人牙周膜细胞(PDLC)碱性磷酸酶(ALP)的活性及其表达的影响。方法采用细胞培养技术分离PDLC,对照组为仅含1%胎牛血清的细胞培养液,实验组分别加入不同浓度的氧化苦参碱溶液和Pg-LPS。采用细胞培养技术和酶联免疫、实时荧光定量RT-PCR技术,观察Pg-LPS对PDLC的ALP活性及其表达的影响。结果在25μg/mL的Pg-LPS作用下,PDLC的ALP活性及其表达明显减低,加入一定浓度的氧化苦参碱溶液后,PDLC的ALP活性及其表达有一定程度恢复。结论氧化苦参碱可以减轻Pg-LPS对PDLC的破坏,对Pg-LPS抑制PDLC的ALP活性及其表达的现象具有拮抗作用。更多还原

【Abstract】 Objective To study the effect of oxymatrine on the activity of alkaline phosphatase(ALP)and the expression of human periodontal ligament cells(PDLC)stimulated by Porphyromonas gingivalis lipopolysaccharide(Pg-LPS). Method The human PDLC were isolated by cell culture technique. The control group was treated with 1% fetal bovine serum. The experimental groups were treated with different concentrations of oxymatrine solution and Pg-LPS. The activity of ALP and its expression in human PDLC were observed by cell culture and enzyme-linked immunosorbent assay and real time PCR. Results The activity and expression of ALP in human PDLC were significantly decreased at 25 μg/mL Pg-LPS. After addition of oxymatrine solution at a certain concentration,the ALP activity and expression had a certain degree of recovery. Conclusion Oxymatrine can reduce the damage of Pg-LPS on human PDLC and raise the ALP activity and its expression.更多还原